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Of note, the commercial Iscove’s Modified Dulbecco’s Medium (IMDM) and Serum Free Expansion Medium (SFEM), which were used as the basal medium for erythroblast cultures, contain a supraphysiological concentration of cystine at 292 μM as compared to physiological plasma concentration of 40–75 μM cysteine in SCD patients, which decreased further to 30–60 μM during vaso-occlusive pain episodes 36

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