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To evaluate the endothelium-dependent contraction to ACh only, vessels with intact endothelium can be incubated with L-NAME and/or EDH inhibitors [please see Table 15, and section titled “Endothelium-dependent hyperpolarization (EDH)”] to prevent the biphasic response and optimize endothelium-dependent contractions (476)

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The models differ with regard to the following aspects: the animal species utilized, the route of administration of the agent, the protocol for both induction and elicitation of responses, type of response measured, and judgment of significant response