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For SDS-PAGE and Western blotting studies, SEC-purified shelterin was diluted to 0.9 µM and incubated with increasing concentrations of compound 40 or 60 (negative control) in the same range as for the MP experiment (from 0 to 300 µM)

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A protocol calling for 5mg intranasal (approximately 2.5 sprays at 2mg/spray) forces the researcher to choose between 2 sprays (4mg actual dose) or 3 sprays (6mg actual dose)

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