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For cell proliferation assays with fold change in cell number, cells were cultured in replicates of 3 in 96-well plates at 1000 (suspension) or 150 (adherent) cells per well in 200 uL RPMI or DMEM base media under the conditions described in each experiment, and a separate group of 3 wells was also plated for each cell line with no treatment for an initial time point

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Coating synthetic NPs cores with natural erythrocyte membranes endows these carriers with “self” identifiers like CD47, thus evading phagocytic clearance