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RNA Isolation and Quantitative Real-Time PCR Total RNA was extracted from mouse islets or INS-1 cells with TRIzol reagent (Invitrogen, Carlsbad, CA) and reversely transcribed into cDNA using a reverse-transcription kit (Invitrogen)

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Blake's co-defendant 31-year-old Emily Anna-Lee Sturgis entered a guilty plea in July to charges of conspiracy and bank fraud

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