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Consistent with the EAAT activity data, differentiation was associated with the induction of SCL1A3 , with a more pronounced effect in the presence of Glu, suggesting EAAT1 involvement in Glu uptake by OCLs ( P = 0.0273, two-sided Student’s t -test for paired data) (Fig

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General methodology: Briefly, sample solution (0.5 mL) is mixed with 0.5 mL phosphate buffer (0.2 M, pH 6.6) and 0.5 mL potassium ferricyanide (1%)