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Overnight cultures were diluted 100-fold into fresh broth and incubated at 200 rpm for 3.5 h to reach their exponential growth stage (absorbance at 600 nm, OD 600 , of 0.24), at which time HgCl 2 (0, 10, or 50 μM), SH-SAMMS (100 μg/mL), or Hg–S–SAMMS (100 μg/mL SH-SAMMS with 1.2 mmol Hg(II)/g loaded) were added to replicate 10 mL aliquots of the Hg-sensitive and Hg-resistant bacterial cultures

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Lysosomalprotective protein 20 kDa chain]."