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To assess replication fork dynamics in these cells, we used the well-established DNA fiber assay based on pulse labeling of cells with the halogenated thymidine analogues 5-chloro-2′-deoxyuridine (CldU) and 5-iodo-2′-deoxyuridine (IdU), followed by visualization of labeled replication tracts on DNA fiber spreads by indirect immunofluorescence

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