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Viral infection For lentiviral production, HEK293T cells were co-transfected with 4 μg Δ8.91, 1 μg pVSVG, 5 μg of lentivector (shRNA targeting SHMT2 : CCAGAATTTGTAGCTGAGATT, and ATF4 : GCGAGTGTAAGGAGCTAGAAA, obtained from RNA Technology platform and Gene manipulating core, Academia Sinica) by using 30 μL of Turbofect (Thermo, R0533) in 1 mL of Opti-MEM (Gibco, 31985070) according to the manufacturer’s instructions

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Notably, this cultivar-biased late activation was independently supported by comparison with the ME-responsive cultivar Gobernadora, in which several of the same thiol–redox genes were previously reported as upregulated during late induction (Bélanger et al., 2018), providing cross-study validation of the observed patterns