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BioLock (IBA Life Sciences) and 1:10 (v:v) 10x Buffer W (1.0 M Tris-HCl, 1.5 M NaCl, 10 mM EDTA, pH 8.0) was added to the filtered 293F supernatant and flowed (0.5–1.0 mL/min) over a Strep-TactinXT (IBA life sciences) column at 4 °C

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At high doses, oxidative stress and formation of toxic metabolites mediated by CYP2E1 are known as the principal pathway of toxicity induced by alcohol (ALC) and acetaminophen (APAP) 1,2,3,4