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Further studies showed that NNMT can regulate Sirt1 expression and the stability of Sirt1 protein by lowering Sirt1 ubiquitination ( NNMT knockdown significantly decreased glucose consumption and the expression of glycolysis-related enzymes, including HK2, LDHA, and PAGM1, thereby increasing the 5-FU sensitivity of EC1 cells ( PGAM1 upregulated 3-phosphoglycerate (3-PG) and 2-PG levels, inhibiting aerobic glycolysis ( Owing to high NNMT expression, glucose-restricted ovarian cancer cells can acquire metabolic adaptations, using other sugars and some methylated substrates to feed their metabolism