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[3] [5] Topical application also keeps the copper load overwhelmingly local: intact stratum corneum is a substantial barrier to a ~403 Da charged metal complex, which limits systemic copper exposure — the same barrier property that limits topical efficacy is what limits topical copper risk

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Both the 4.5% acrylamide stacking gel and the 13.3% acrylamide-SDS gel were prepared using a stock solution containing 38.67% ( wt / wt ) acrylamide and 1.33% ( wt / wt ) N , N -bis-(methylene acrylamide)