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To obtain nuclear lysate, cells were collected using cellular lysis buffer (5 mM HEPES pH 8.0, 5 mM KCl, 0.5% v/v IGEPAL) on ice during 15 min

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Pathogenesis of Inflammation Since the brain does not mount classical allergic reactions and IgE cannot cross the blood-brain barrier (BBB), it is therefore unsurprising that mast cells, although distributed systemically, normally lack surface expression of the high-affinity IgE receptor (FcεRI)