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The Subclinical Presentation Most of my post-weight-loss patients who test positive for B12 deficiency on their first blood panel do not have florid symptoms

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Cells were then lysed in lysis buffer [1.28 M sucrose, 40 mM Tris-HCl (pH 7.5), 20 mM MgCl 2 , 4% (v/v) Triton X-100]