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Silencing the expression of NCOA4 by RNAi knockdown significantly inhibited ferritinophagy, thereby suppressing ferroptosis in mouse embryonic fibroblasts (Gao et al., 2016)

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Annealed gRNAs were cloned into LentiCRISPRv2-RFP670 (Addgene plasmid # 187646) at a 1:5 vector to insert ratio by Gibson assembly using the ClonExpress Ultra One Step Cloning Kit, transformed in 10-beta Competent E