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Thus, application of substance P and other cationic secretagogues transiently increases intracellular Ca 2+ concentrations through G protein-mediated activation of phospholipase C (PLC), and pretreatment of mast cells with pertussis toxin abolishes both inositol-3-phosphate production and peptide-induced mast cell degranulation (Mousli et al., 1989, 1995