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To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (GenoGrinder 2000 by the supplier Glen Mills) followed by centrifugation

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In addition to the well-established SORT for modulating LNP, recent preclinical practices have demonstrated that precise tuning of lipid component proportions within LNP formulations, for instance, Rampado et al