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(a) Immunoblot (left) and RT-qPCR (right) to measure levels of methionine synthase (encoded by Mtr) in the colon and stomach of WT (n = 4, 3 M 1 F) and OSKM (n = 8, 4 M 4 F) mice from Fig

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The National Academy of Medicine set no official upper limit for B12 because no clear toxicity threshold has been established in human studies B12 is extremely difficult to overdose on orally because only 1.5-2 mcg per meal is actively absorbed, and excess is excreted through the kidneys Unexpectedly high serum B12 without supplementation is a red flag that warrants medical investigation, not a sign of good health The Brasky et al