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Peptides were then separated over 100 μm × 15 cm ReproSil-Pur C18-AQ 1.5-µm silica beads (Beijing Qinglian Biotech Co., Ltd, Beijing, China), at a flow rate of 300 nL/min using a gradient: 0–4 min (5–10% B), 4–46 min (10–24% B), 46–53 min (36% B), 53–54 min (95% B), 54–60 (95% B)

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How Peptides Influence Muscle Growth Signalling In biological research models, many peptides associated with muscle growth are studied for their ability to influence the hypothalamic-pituitary axis , a regulatory system responsible for hormone signalling throughout the body