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In uncertain cases, additional tests may include: Methylmalonic acid (MMA) and homocysteine levels (elevated in B12 deficiency) Full blood count (may show macrocytic anaemia) Folate levels (deficiency can present similarly to B12 deficiency) Intrinsic factor and parietal cell antibodies (to diagnose pernicious anaemia) Coeliac disease screening if clinically indicated Treatment approaches: For vitamin D deficiency , treatment depends on severity

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