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While hydroxyl radicals induce mitochondrial oxidative stress, the reactive copper intermediates covalently bind to DLAT, triggering its aggregation and thereby initiating cuproptosis

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Mac-CF ( Cftr fl10/fl10 Cx3cr1 cre/+ ) and the sibling congenic control (Ctrl) ( Cftr fl10/fl10 Cx3cr1 +/+ ) mice were screened by PCR using the genotyping primers ( Table 1 )