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After 5 min incubation at room temperature, the following reagents were added: 100 µL of 38% formaldehyde (only to blank samples) and 500 µL of 0.2 M ferric nitrate reagent (Sigma-Aldrich, Darmstadt, Germany)

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B3 that is not methylated can be instead transformed into another molecule called nicotinamide adenine dinucleotide (NAD+), which is essential for producing energy, repairing DNA, and other benefits