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After the hybridization, samples were washed once for 5 min in 4[×[SSC at 80 °C, three times for 20 min each in 2[×[SSC and 50% formamide at 80 °C, three times for 40 min each in 0.1[×[SSC at 80 °C, and once for 5 min in PBS containing 0.5% Triton X-100 (PBT) at RT