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The most substantial evidence comes from a 2010 randomized controlled trial involving 40 healthy adults who received intravenous doses ranging from 42 to 1,260 mg of thymosin beta 4

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Few ideal properties of the drug that can be administered transdermally require that drug should be essentially non-irritating, dose should be preferably up to 20 mg or less and partition coefficient in octanol / water should be between 1 to 4