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Crystallization and structure determination of GluK3 LBD with SYM GluK3 LBD S1S2 domain from residues N402-K515 of S1 domain linked by GT linker to P638-P775 of S2 domain (numbering according to mature polypeptide) was cloned into a pET22b vector with an N-terminal hexahistidine tag (MHHHHHH) followed by LVPRGS thrombin cleavage site

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[DOI] [PMC free article] [PubMed] [Google Scholar] 198.Hammouda M., Ford A., Liu Y., Zhang J