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A 20 μl mix, containing 8 μl of cDNA sample, 10 μl of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 μl of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)

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PD-L1 monoclonal antibody-conjugated nanoparticles enhance drug delivery level and chemotherapy efficacy in gastric cancer cells