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Insulin-like growth factor-I affects the regulation of GH secretion at the hypothalamus by inhibiting the GHRH gene expression (19) and stimulating somatostatin secretion (20), whereas at the pituitary, IGF-I inhibits spontaneous and GHRH-stimulated GH secretion (3)

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It includes cleaning the vial septum with alcohol before each access, using sterile needles, and maintaining clean conditions