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Materials for Detecting Substrate Depletion from AlphaScreen Assays Medium-binding white solid-bottom 1536-well plates Plate reader capable of 1536-well AlphaScreen detection (such as the EnVision Plate Reader equipped with a 1536 HTS AlphaScreen aperture) Assay Buffer (PBS pH 7.4, 0.01% Tween-20 or another preferred buffer, see Note 1 ) Enzyme at double the final concentration in assay buffer Substrate at four-fold the final concentration in assay buffer Mixture of nickel chelate acceptor beads (20 μg/mL working stock for 5 μg/mL, final) and streptavidin donor beads (20 μg/mL working stock for 5 μg/mL, final) suspended in assay buffer (or beads conjugated for the preferred specificity, such as anti-GST) ( see Note 2 ) Experimental compounds suspended in DMSO (stock concentrations of 640 nM – 10,000 μM for a working concentration range of 3.7 nM – 57.5 μM after a 174-fold dilution into the assay mixture) Liquid transfer pin tool (such as a Kalypsys automated pin-tool station) ( see Note 3 ) Centrifuge equipped with a rotor that will accommodate plates Liquid dispenser capable of delivering small volumes (such as a BioRAPTR Flying Reagent Dispenser) 2.2
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Lamberts L-Carnitine 500mg - 60 caps:Lamberts L-Carnitine 500mg - 60 caps
It is commonly investigated for its effects on muscle growth, fat loss, anti-aging, and recovery, as it promotes protein synthesis and cellular repair
