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Various instances of this phenomenon are demonstrated, including the DedA superfamily proteins (TMEM41B and VMP1), the Hop1, Rev7 and Mad2 (HORMA)-domain-containing proteins (ATG13 and ATG101), transmembrane segment of ATG9, chorein-N domain at the N termini of lipid transfer proteins (ATG2), and ubiquitin-like ATG conjugation systems