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Now, we expressed the recombinant rat MGST1 containing a six-histidine-tag at the N-terminus in C43 (DE3) strain (Lucigen), which gives a higher yield than BL21 (DE3) pLys SL strain used previously

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To ensure consistent peptide concentrations across samples, the digested peptides were quantified using the Pierce Quantitative Colorimetric Peptide Assay (#23275, Thermo Fisher Scientific), a modified BCA assay