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In humans, two enzymatic reactions are dependent on vitamin B 12 —methylmalonyl coenzyme A mutase (MUT) reaction and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) reaction (Fig 2)

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The increased intracellular levels of Ca 2+ that are induced by binding of ghrelin to GHSR activate diacylglycerol lipase-α, the enzyme that synthesizes 2-arachidonoylglycerol (an endogenous cannabinoid), directly or through activation of PKC, resulting in increased 2-arachidonoylglycerol synthesis and release in the hypothalamus 191