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In published preclinical and mechanistic research contexts, AHK-Cu has been studied for its role in cell signaling pathways associated with fibroblast activity, dermal papilla cell behavior, collagen-related gene expression, angiogenic signaling, oxidative stress response, and tissue remodeling mechanisms

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It is used in the treatment and prevention of vitamin B12 deficiency in patients with conditions such as: Addisonian (pernicious) anemia Malabsorption Atrophic gastritis Prolonged treatment with metformin Small bowel bacterial overgrowth (SIBO) Total or partial gastrectomy Infection with the fish parasite Diphyllobothrium latum Helicobacter pylori infection Pancreatic dysfunction Pancreatic or bowel malignancy Dietary deficiency (e.g