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564 HO-1 and GPX4 were downregulated due to Nrf2 defects in astrocytes, leading to increased mitochondrial fragmentation, DNA oxidation, and lipid peroxidation in mouse astrocytes, 564 suggesting that Nrf2 deficiency promotes ferroptosis in astrocytes in AD mice

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The supernatant is transferred to a new centrifuge tube and stored at -80 °C for later use