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Cells were then treated (duplicate wells/treatment) with stuarosporine (1 μM) for 24 h [21] or H 2 O 2 (100–2500 μM, Sigma-Aldrich) for 2 h at 37 °C ± a 30 min pre-treatment with inhibitors of cell death: liproxstatin (LPX, 10 µM, Sigma-Aldrich) and necrostatin-1 (25 µM) [22]