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Camacho-Pereira and colleagues (Cell Metabolism, 2016) reported that CD38 expression and NADase activity rise with age in mice and drive tissue NAD+ decline alongside SIRT3-dependent mitochondrial dysfunction, while Liu and colleagues (Cell Metabolism, 2018) applied stable-isotope tracing to quantify NAD synthesis and breakdown fluxes directly and map tissue-specific turnover rather than inferring it from pool sizes