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In summary, the results presented demonstrate the efficiency of the GST-His purification for the purification of S

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After the electrophoretic run, proteins were transferred to a nitrocellulose membrane using iBlot2 Gel transfer Device and blocked in 0.1% TBS-Tween20 and 5% (w/v) bovine serum albumin (BSA) for 1 hour at room temperature (RT)