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Similar to MTT, the resazurin assays are a good indicator of cell viability, but not specific for mitochondrial activity evaluation [288]

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However, through electron microscopy, the researchers found a surprising result: inhibition of cAMP, instead of suppressing synaptic vesicles, caused an increased supply of synaptic vesicles from the readily released pools in the active zone by PKA-mediated phosphorylation of synapsin, leading to increased release of glutamate induced by high-frequency action potentials