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Briefly, cells were seeded at 2 × 10 5 cells per well on a 96-well microplate and cultured with or without IGF-1(100 ng ml −1 ) for 24 h to allow BrdU incorporation

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35,62 It is likewise known that the DAergic cell toxin 6-hydroxydopamine (6-OHDA) exerts its toxicity through oxidative stress, as do Aβ and iron (Fe 2+ )