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The various isoforms, ZO-1, ZO-2, and ZO-3, are all characterized by their ability to interact with different cellular proteins through a multitude of protein binding domains, such as the SH3 domain, the PDZ domain, and the leucine-zipper domain, 48,49 and are also essential for scaffold formation and the connection of other tight junction proteins to the cytoskeleton

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Hydration plays a role too, so consider drinking plenty of fluids before your appointment