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A high efficiency consistent with the principles of green chemistry allows the careful use of natural resources and the mitigation of nano-waste generation ( Zingiber officinale extract, which demonstrated an efficient green synthesis approach that produces bioactive functionalized AgNPs possessing the ability to act against Bacillus subtilis ( 3.2 Characterization of AgNPs To examine colloidal stability, the UV–vis spectra, bandwidth (Δλ), and resonance maximum wavelength (λmax) of AgNPs were measured after each 3rd day of the AgNP solution, as shown in Supplementary Figure S2