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SEC-SAXS data for the ChiLob7/4 and SAP1.3 variants as IgG displayed the same trends in R g , D max , and Kratky plots as the F(ab’) 2 fragments (i.e., C232S κC214S was the least deviated from a Gaussian distribution and C232S + C233S the most), indicating that the conformational differences are retained in whole IgG, with the observed effects independent of the Fc (Supplementary Figs