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Membranes were blocked with 5% (wt/vol) nonfat dried milk in TBS containing 0.1% Tween 20 (TBST) and subsequently incubated with the appropriate antibodies in 5% (wt/vol) nonfat dried milk in TBST overnight at 4 °C

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Women who experience very heavy, painful periods may be showing signs of endometriosis, a condition where tissues usually found in the womb are present elsewhere in the body