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Because the presynaptic blockade of noradrenalin release from vasoconstrictor nerve fibers does not completely eliminate the vessel spasm stimulated by low temperatures and the construction of a vascular bed is also observed in the internal organs, it is suggested that other nonadrenergic mechanisms could be involved in this process (oxidative pathway) [60, 61]

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The l-EVs were prepared from culture media of HEK293 cells expressing Halo7-MIM I-BAR and labeled with SaraFluor 650T-conjugated Halo ligand as described above