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Differences between analyses of whole tissue, which typically report GSSG contents corresponding to overall concentrations in the micromolar range, and roGFP analyses of cytosolic redox potential, which suggest much lower GSSG concentrations in compartments such as the cytosol, could be reconciled if a significant amount of GSSG is present in compartments such as the endoplasmic reticulum, vacuole, or apoplast, where glutathione reduction capacity is relatively low