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Briefly, 200 mg liver tissue was taken en bloc from mice injected with the TRAP construct and from Rosa LSL-GFP-L10a mice, homogenized with lysis buffer, and incubated with magnetic beads that were conjugated with anti-GFP antibodies (clones Htz-GFP-19F7 and Htz-GFP-19C8, Memorial Sloan-Kettering Monoclonal Antibody Facility, New York, New York, USA) to affinity purify RNA that was bound by the GFP-L10a fusion protein

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These models often fail to capture the long-term biodistribution, degradation behavior, and potential toxicity of nanoparticles after multiple applications